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Article

Using the E1A Minigene Tool to Study mRNA Splicing Changes.

Journal of visualized experiments : JoVE - 22 Apr 2021

Basei Fernanda L, Moura Livia A R, Kobarg Jörg

Abstract excerpt

mRNA processing involves multiple simultaneous steps to prepare mRNA for translation, such as 5´capping, poly-A addition and splicing. Besides constitutive splicing, alternative mRNA splicing allows the expression of multifunctional proteins from one gene. As interactome studies are generally the first analysis for new or unknown proteins, the association of the bait protein with splicing factors is an indication...

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PMID: 33970143
DOI: 10.3791/62181

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