Article

Protocol for a minigene splice assay using the pET01 vector

2025-02-04

Abstract excerpt

<h4>Summary</h4> Aberrant splicing is recognized as a key contributor of hereditary disorders, yet characterizing the molecular effects of splice variants is an important task that poses several challenges. Here, we present a protocol for an in vitro splice assay using a minigene approach, which is especially useful when patient samples are not available for RNA analysis or when target genes or isoforms are not...

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Literature Corpus work: 727d555a-8a46-5fec-bc8f-eddffc9a3fde
DOI: 10.1101/2025.02.03.636202

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