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Article

Development of simple and efficient in planta transformation method for rice (Oryza sativa L.) using Agrobacterium tumefaciens.

Journal of bioscience and bioengineering - 1 Oct 2005

Supartana Putu, Shimizu Tsutomu, Shioiri Hidenari, Nogawa Masahiro, Nozue Masayuki, Kojima Mineo

Abstract excerpt

Seeds of rice (Oryza sativa L. var. Koshihikari) were soaked in water for 2 d. Thereafter, the embryo containing an apical meristem was inoculated with Agrobacterium tumefaciens by piercing a site of the husk overlying the embryonic apical meristem with a needle that had been dipped in an A. tumefaciens inoculum. The inoculated seeds were then grown to maturation (T0 plants) and allowed to pollinate naturally to...

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PMID: 16310727
DOI: 10.1263/jbb.100.391

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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