Article
Identification of pathogenicity-related genes in the rice pathogen Ustilaginoidea virens through random insertional mutagenesis.
Fungal genetics and biology : FG & B - 1 Mar 2015
Yu Mina, Yu Junjie, Hu Jiankun, Huang Lei, Wang Yahui, Yin Xiaole, Nie Yafeng, Meng Xiangkun, Wang Weiduo, Liu Yongfeng
Abstract excerpt
Agrobacterium tumefaciens-mediated transformation (ATMT) is becoming a popular effective system as an insertional mutagenesis tool in filamentous fungi. To gain more insight into the cellular and molecular mechanisms in the pathogenesis of Ustilaginoidea virens, the causal agent of rice false smut disease, a T-DNA insertion mutant library of U. virens was established using ATMT. We optimized a range of conditions...
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