Article
Development of a facile genetic transformation system for the Spanish elite rice paella genotype Bomba.
Transgenic research - 1 Jun 2022
Saba-Mayoral Andrea, Bassie Ludovic, Christou Paul, Capell Teresa
Abstract excerpt
We report the development of an efficient and reproducible genetic transformation system for the recalcitrant Spanish elite rice paella genotype, Bomba. Preconditioned embryos derived from dry seeds were bombarded with gold particles carrying a plasmid containing a screenable and a selectable marker. We confirmed integration and expression of hpt and gusA in the rice genome. Transformation frequency was ca: 10%...
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