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Genotype-specific optimization of in vitro regeneration and Agrobacterium-mediated transformation in indica rice with 35S:RUBY and CRISPR/LbCas12a system.

Planta - 24 Apr 2026

Sarki Yogita N, Keot Ajay Kumar, Marwein Riwandahun, Singha Dhanawantari L, Gogoi Dhrubo Jyoti, Velmurugan Natarajan, Chikkaputtaiah Channakeshavaiah

Abstract excerpt

MAIN CONCLUSION: This study establishes a genotype-specific transformation system for indica rice cultivars Ranjit, Mahsuri, and Kon Joha using 35S:RUBY and CRISPR/LbCas12a constructs, enabling functional genomics studies and genetic improvement. The indica rice subspecies generally faces challenges in functional genomics and genetic improvement due to its recalcitrance to tissue culture and...

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PMID: 42029959
DOI: 10.1007/s00425-026-05010-1

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