Expression of wild-type and mutant p53 proteins by recombinant vaccinia viruses.

doi:10.1093/nar/20.13.3435

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Article

Expression of wild-type and mutant p53 proteins by recombinant vaccinia viruses.

Nucleic acids research - 11 Jul 1992

Ronen D, Teitz Y, Goldfinger N, Rotter V

Abstract excerpt

To facilitate the purification of wild type p53 protein, we established a recombinant p53 vaccinia viral expression system. Using this efficient eukaryotic expression vector, we found that the expressed p53 proteins retained their specific structural characteristics. A comparison between wild typ...

Topics

Antigens, Viral, Tumor
Cloning, Molecular
Fluorescent Antibody Technique
HeLa Cells
Humans
Mutation
Recombinant Proteins
Tumor Suppressor Protein p53
Vaccinia virus

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