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Article

Expression of the catalytic subunit of human DNA polymerase delta in mammalian cells using a vaccinia virus vector system.

The Journal of biological chemistry - 7 Apr 1995

Zhang P, Frugulhetti I, Jiang Y, Holt G L, Condit R C, Lee M Y

Abstract excerpt

The catalytic polypeptide of human DNA polymerase delta was overexpressed in BSC-40 cells (African green monkey kidney cell line) using the vaccinia virus/pTM1 system. The recombinant human DNA polymerase delta was purified to homogeneity in two steps using an immunoaffinity column and a single-stranded DNA-cellulose column. Levels of expression were about 1% of soluble cytosolic protein. The recombinant...

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PMID: 7713899
DOI: 10.1074/jbc.270.14.7993

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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