Article
Expression of the catalytic subunit of human DNA polymerase delta in mammalian cells using a vaccinia virus vector system.
The Journal of biological chemistry - 7 Apr 1995
Zhang P, Frugulhetti I, Jiang Y, Holt G L, Condit R C, Lee M Y
Abstract excerpt
The catalytic polypeptide of human DNA polymerase delta was overexpressed in BSC-40 cells (African green monkey kidney cell line) using the vaccinia virus/pTM1 system. The recombinant human DNA polymerase delta was purified to homogeneity in two steps using an immunoaffinity column and a single-s...
Topics
- Animals
- Base Sequence
- Catalysis
- Cell Line
- Chlorocebus aethiops
- Cloning, Molecular
- DNA Polymerase III
- DNA Primers
- DNA-Directed DNA Polymerase
- Genetic Vectors
- Humans
- Molecular Sequence Data
- Mutation
- Recombinant Proteins
- Vaccinia virus