Article
Genes expressed in Pseudomonas putida during colonization of a plant-pathogenic fungus.
Applied and environmental microbiology - 1 Jul 2000
Lee S W, Cooksey D A
Abstract excerpt
In vivo expression technology (IVET) was employed to study colonization of Phytophthora parasitica by a biological control bacterium, Pseudomonas putida 06909, based on a new selection marker. The pyrB gene, which encodes aspartate transcarbamoylase, an enzyme used for pyrimidine biosynthesis, was cloned from P. putida 06909. A pyrB-disrupted mutant did not grow in pyrimidine-deficient media unless it was...
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