Constraining the expression of nicotinic acetylcholine receptors by using pentameric constructs.

Abstract excerpt

Much of our understanding of ligand-gated ion channels comes from heterologous expression studies. However, this technique cannot produce receptors with a predetermined subunit composition for channels formed by several different subunits and cannot insert a single mutation copy if the subunit of interest is present in several copies in the channel. Here, we describe a novel approach that overcomes these problems...

Topics

• Animals
• Humans
• Mutation
• Oocytes
• Protein Engineering
• Protein Subunits
• Receptors, Nicotinic
• Xenopus laevis