Article
[Complete HBV DNA clone and sequence from serum samples of severe hepatitis B patients].
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology - 1 Oct 2005
Wu Wei, Li Lan-juan, Chen Yu, Li Jun, Qian Xiang, Cheng Dong-qing
Abstract excerpt
OBJECTIVE: To study the association between hepatitis B virus (HBV) mutants and the pathogenesis of severe hepatitis B by full-length HBV genome. METHODS: Serum samples from 10 severe hepatitis B patients were collected in our hospital. Serum HBV DNAs were extracted using DNA mini Kit, and amplified by LA Taq DNA polymerase to yield full-length HBV DNA. PCR products were isolated and cloned into vector pUCm-T,...
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