Article
Effect of an amino acid insertion into the omega loop region of a class C beta-lactamase on its substrate specificity.
Biochemistry - 21 Jul 1998
Nukaga M, Taniguchi K, Washio Y, Sawai T
Abstract excerpt
The extended-substrate specificity of Enterobacter cloacae GC1 beta-lactamase is entirely due to a three amino acid insertion after position 207. To clarify the reason for the extended-substrate specificity, Ala, Ala-Ala, Ala-Ala-Ala, and Ala-Ala-Ala-Ala were inserted after position 207 on the ba...
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- Aztreonam
- Boronic Acids
- Enterobacter cloacae
- Enzyme Activation
- Enzyme Stability
- Hot Temperature
- Kinetics
- Microbial Sensitivity Tests
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Protein Structure, Secondary
- Substrate Specificity
- Sulbactam
- beta-Lactamase Inhibitors