Article
Preparation of human metabolites of propranolol using laboratory-evolved bacterial cytochromes P450.
Biotechnology and bioengineering - 20 Feb 2006
Otey Christopher R, Bandara Geethani, Lalonde James, Takahashi Katsuyuki, Arnold Frances H
Abstract excerpt
Testing the toxicities and biological activities of the human metabolites of drugs is important for development of safe and effective pharmaceuticals. Producing these metabolites using human cytochrome P450s is difficult, however, because the human enzymes are costly, poorly stable, and slow. We have used directed evolution to generate variants of P450 BM3 from Bacillus megaterium that function via the "peroxide...
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