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Article

Exploring PTDH-P450BM3 Variants for the Synthesis of Drug Metabolites.

Chembiochem : a European journal of chemical biology - 16 Feb 2018

Beyer Nina, Kulig Justyna K, Fraaije Marco W, Hayes Martin A, Janssen Dick B

Abstract excerpt

The conversion of a series of pharmaceutical compounds was examined with three variants of cytochrome P450BM3 fused to phosphite dehydrogenase (PTDH) to enable cofactor recycling. Conditions for enzyme production were optimized, and the purified PTDH-P450BM3 variants were tested against 32 commercial drugs by using rapid UPLC-MS analysis. The sets of mutations (R47L/F87V/L188Q and R47L/F87V/L188Q/E267V/G415S)...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 29181885
DOI: 10.1002/cbic.201700470

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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