Article
Different real time PCR approaches for the fine quantification of SNP's alleles in DNA pools: assays development, characterization and pre-validation.
Journal of biochemistry and molecular biology - 30 Sept 2005
Mattarucchi Elia, Marsoni Milena, Binelli Giorgio, Passi Alberto, Lo Curto Francesco, Pasquali Francesco, Porta Giovanni
Abstract excerpt
Single nucleotide polymorphisms (SNPs) are becoming the most common type of markers used in genetic analysis. In the present report a SNP has been chosen to test the applicability of Real Time PCR to discriminate and quantify SNPs alleles on DNA pools. Amplification Refractory Mutation System (ARMS) and Mismatch Amplification Mutation Assay (MAMA) has been applied. Each assay has been pre-validated testing...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
