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PCR-based SNP genotyping: A comprehensive comparison of methods for affordable and accurate detection of class IV mutations.

Analytica chimica acta - 8 Jun 2025

Muneeswaran Kajan, de Silva Varuni A, Branavan Umayal, Dayabandara Madhubhashinee, Hanwella Raveen, Chandrasekharan Naduviladath Vishvanath

Abstract excerpt

Single nucleotide polymorphisms (SNPs) influence gene function and impact health and disease. Identifying and genotyping SNPs is crucial in various areas of research and applications. This study compared five PCR-based methods for detecting a challenging T-to-A SNP, rs9939609: ARMS-PCR, PIRA-PCR, TaqMan qPCR, CADMA with HRM, and HRM, using snapback primers. Sanger sequencing served as the gold standard. Five...

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PMID: 40253069
DOI: 10.1016/j.aca.2025.343994

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