Article
Multilocus markers for mouse genome analysis: PCR amplification based on single primers of arbitrary nucleotide sequence.
Mammalian genome : official journal of the International Mammalian Genome Society - 1 Jan 1992
Nadeau J H, Bedigian H G, Bouchard G, Denial T, Kosowsky M, Norberg R, Pugh S, Sargeant E, Turner R, Paigen B
Abstract excerpt
Polymerase chain reaction (PCR) based on single primers of arbitrary nucleotide sequence provides a powerful marker system for genome analysis because each primer amplifies multiple products, and cloning, sequencing, and hybridization are not required. We have evaluated this typing system for the mouse by identifying optimal PCR conditions; characterizing effects of GC content, primer length, and multiplexed...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.