Article
High-resolution typing for chicken BF2 (MHC class I) alleles by automated sequencing.
Animal genetics - 1 Oct 2005
Livant E J, Ewald S J
Abstract excerpt
Sequence-based typing (SBT) was developed for major histocompatibility complex (MHC) class I and class II alleles in humans. We report here the development and application of a SBT method for alleles of the chicken BF2 locus (the more polymorphic of the two MHC class I loci in chickens). Exon 2 of the BF2 gene was selectively amplified from genomic DNA using a BF2 locus-specific PCR primer. Exon 2 sequences were...
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