Article
Extensive analysis of different allelelic structures of the chicken BF2 and beta2m proteins.
Veterinary immunology and immunopathology - 15 Sept 2006
Li Xin Sheng, Fang Qin Mei, Yan Ruo Qian, Gao Feng Shan, Hao Hui Fang, Jia Zhen Hu, Lin Chang You, Xia Chun
Abstract excerpt
No information is available to date on the different allelelic structures of the chicken MHC class I (BF2) and beta2m proteins. To elucidate the structure, new allelic beta2m and five different BF2 genes were expressed solubly and purified in a pMAL-p2X/E. coli TB1 system. The 2D structure was detected by circular dichroism (CD) spectroscopy, and the 3D structures of their peptide-binding domain (PBD) were...
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