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Article

A high-throughput method for screening of rapamycin-producing strains of Streptomyces hygroscopicus by cultivation in 96-well microtiter plates.

Biotechnology letters - 1 Aug 2005

Xu Zhi-nan, Shen Wen-he, Chen Xi-yang, Lin Jian-ping, Cen Pei-lin

Abstract excerpt

A novel high-throughput cultivation method was developed to rapidly screen large numbers of rapamycin-producing mutants of Streptomyces hygroscopicus by duplicate culturing of isolates on the surfaces of agar-solidified 96 wells in microtiter plates. One copy of the cultures was used for the rapamycin bioassay and the other identical copy, representing potentially high yielding strains, was preserved for further...

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PMID: 16132865
DOI: 10.1007/s10529-005-8463-y

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