Article
Oxytetracycline production in solid state and submerged fermentation by protoplast fusants of Streptomyces rimosus.
Proceedings of the National Science Council, Republic of China. Part B, Life sciences - 1 Feb 1991
Yang S S, Kao C Y
Abstract excerpt
Streptomyces rimosus TM-55 was treated with 3% EMS, and 29 auxotrophic mutants (AM-1 - AM-29) were isolated from 5457 colonies at a survival rate between 6.6-66.7%. Three sets of the auxotrophic mutants, AM-3 and AM-27, AM-7 and AM-28, and AM-3 and AM-21, were chosen for protoplast fusion with 50% PEG 1000 for 30 minutes at 25 degrees C, and 25 fusants were isolated (f-1 - f-25). In solid substrate,...
Topics
- Fermentation
- Membrane Fusion
- Mutation
- Oxytetracycline
- Polyethylene Glycols
- Protoplasts
- Streptomyces