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Article

Construction and evaluation of a O139 Vibrio cholerae vaccine candidate based on a hemA gene mutation.

Vaccine - 1 May 2006

Ravichandran Manickam, Ali Syed Atif, Rashid Nur Haslindawaty Abdul, Kurunathan Sinniah, Yean Chan Yean, Ting Lai Chin, Bakar Afifi Sheikh Abu, Lalitha Pattabiraman, Zainuddin Zainul F

Abstract excerpt

In this paper, we describe the development of VCUSM2, a live metabolic auxotroph of Vibrio cholerae O139. Auxotrophy was achieved by mutating a house keeping gene, hemA, that encodes for glutamyl-tRNA reductase, an important enzyme in the C5 pathway for delta-aminolevulenic acid (ALA) biosynthesis, which renders this strain dependent on exogenous ALA for survival. Experiments using the infant mouse and adult...

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Identifiers

PMID: 16102875
DOI: 10.1016/j.vaccine.2005.07.016

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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