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Development of reverse-transcription PCR techniques to analyse the density and sex ratio of gametocytes in genetically diverse Plasmodium chabaudi infections.

Molecular and biochemical parasitology - 1 Dec 2007

Drew Damien R, Reece Sarah E

Abstract excerpt

We have developed cross-genotype and genotype-specific quantitative reverse-transcription PCR (qRT-PCR) assays to detect and quantify the number of parasites, transmission stages (gametocytes) and male gametocytes in blood stage Plasmodium chabaudi infections. Our cross-genotype assays are reliable, repeatable and generate counts that correlate strongly (R(2)s>90%) with counts expected from blood smears. Our...

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Identifiers

PMID: 17889948
DOI: 10.1016/j.molbiopara.2007.08.004
PMCID: PMC3818572

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