Article
Implications of the mutation S164A on Bacillus subtilis levansucrase product specificity and insights into protein interactions acting upon levan synthesis.
International journal of biological macromolecules - 15 Oct 2020
Ortiz-Soto Maria Elena, Porras-Domínguez Jaime Ricardo, Rodríguez-Alegría María Elena, Morales-Moreno Luis Alberto, Díaz-Vilchis Adelaida, Rudiño-Piñera Enrique, Beltrán-Hernandez Nidia E, Rivera Heriberto Manuel, Seibel Jürgen, López Munguía Agustín
Abstract excerpt
Mutation S164A largely affects the transfructosylation properties of Bacillus subtilis levansucrase (SacB). The variant uses acceptors such as glucose and short levans with an average molecular weight of 7.6 kDa more efficiently than SacB, leading to the enhanced synthesis of medium and high molecular weight polymer and a blasto-oligosaccharide series with a polymerization degree of 2-10. A 3-fold increase in...
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