Article
The nuclear pore complex-associated protein, Mlp2p, binds to the yeast spindle pole body and promotes its efficient assembly.
The Journal of cell biology - 18 Jul 2005
Niepel Mario, Strambio-de-Castillia Caterina, Fasolo Joseph, Chait Brian T, Rout Michael P
Abstract excerpt
The two yeast proteins Mlp1p and Mlp2p (homologues of the vertebrate protein Tpr) are filamentous proteins attached to the nuclear face of nuclear pore complexes. Here we perform a proteomic analysis, which reveals that the two Mlps have strikingly different interacting partners, testifying to their different roles within the cell. We find that Mlp2p binds directly to Spc110p, Spc42p, and Spc29p, which are three...
Topics
- Calmodulin-Binding Proteins
- Cell Nucleus
- Cytokinesis
- Cytoskeletal Proteins
- DNA Polymerase I
- DNA-Directed DNA Polymerase
- Mass Spectrometry
- Metaphase
- Microscopy, Electron, Transmission
- Microtubule-Organizing Center
- Mutation
- Nuclear Pore Complex Proteins