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Article

Biotransformation of beta-ionone by engineered cytochrome P450 BM-3.

Applied microbiology and biotechnology - 1 Mar 2006

Urlacher Vlada B, Makhsumkhanov Akhmadjan, Schmid Rolf D

Abstract excerpt

Wild-type cytochrome P450 monooxygenase from Bacillus megaterium (P450 BM-3) has a low hydroxylation activity for beta-ionone (<1 min(-1)). Substitution of phenylalanine by valine at position 87 led to a more than 100-fold increase in beta-ionone hydroxylation activity (115 min(-1)). Enzyme activity could be further increased by both site-directed and random mutagenesis. The mutant R47L Y51F F87V, designed by...

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Identifiers

PMID: 16001257
DOI: 10.1007/s00253-005-0028-4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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