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Article

Accessibility of nitroxide side chains: absolute Heisenberg exchange rates from power saturation EPR.

Biophysical journal - 1 Sept 2005

Altenbach Christian, Froncisz Wojciech, Hemker Roy, McHaourab Hassane, Hubbell Wayne L

Abstract excerpt

In site-directed spin labeling, the relative solvent accessibility of spin-labeled side chains is taken to be proportional to the Heisenberg exchange rate (W(ex)) of the nitroxide with a paramagnetic reagent in solution. In turn, relative values of W(ex) are determined by continuous wave power saturation methods and expressed as a proportional and dimensionless parameter Pi. In the experiments presented here,...

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Identifiers

PMID: 15994891
DOI: 10.1529/biophysj.105.059063
PMCID: PMC1366712

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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