Article
Orthogonal spin labeling and Gd(III)-nitroxide distance measurements on bacteriophage T4-lysozyme.
The journal of physical chemistry. B - 21 Mar 2013
Garbuio Luca, Bordignon Enrica, Brooks Evan K, Hubbell Wayne L, Jeschke Gunnar, Yulikov Maxim
Abstract excerpt
We present the first example of chemoselective site-specific spin labeling of a monomeric protein with two spectroscopically orthogonal spin labels: a gadolinium(III) chelate complex and a nitroxide radical. A detailed analysis of the performance of two commercially available Gd(III) ligands in t...
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