Article
Genotyping and detection of multiple infections of Toxoplasma gondii using Pyrosequencing.
International journal for parasitology - 1 Aug 2005
Sreekumar C, Hill D E, Miska K B, Vianna M C B, Yan L, Myers R L, Dubey J P
Abstract excerpt
A Pyrosequencing assay, based on SAG2 gene polymorphisms, was designed for genotyping and detection of multiple infections of Toxoplasma gondii. The assay was tested on samples spiked with DNA from single and multiple genotypes of T. gondii and also on a DNA sample from the brain of a rat with multiple infections. To evaluate the comparative efficacy of the assay, identical samples were also analysed by...
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