Article
Determination of genotypes of Toxoplasma gondii strains isolated from patients with toxoplasmosis.
Journal of clinical microbiology - 1 Jun 1997
Howe D K, Honoré S, Derouin F, Sibley L D
Abstract excerpt
To determine the genotypes of Toxoplasma gondii strains associated with human toxoplasmosis, we developed a sensitive approach for typing parasites grown from clinical samples by short-term in vitro culture. A newly described nested PCR assay was capable of amplifying genomic DNA from as few as five parasites in the presence of host tissues. Typing was based on DNA polymorphisms at the SAG2 locus, encoding...
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