Article
Multi-Locus Sequencing for Strain Typing of Toxoplasma gondii.
Journal of visualized experiments : JoVE - 26 May 2026
Khedr Abeer A, Denny Paul W
Abstract excerpt
Toxoplasma gondii is a globally distributed apicomplexan parasite characterized by substantial genetic diversity, necessitating accurate molecular approaches for strain discrimination. The goal of this protocol is to establish a reproducible workflow for targeted multi-locus genotyping of cultured T. gondii isolates. The procedure involves in vitro propagation of representative Type I (RH) and Type II (ME49)...
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