Article
Pyrosequencing, a high-throughput method for detecting single nucleotide polymorphisms in the dihydrofolate reductase and dihydropteroate synthetase genes of Plasmodium falciparum.
Journal of clinical microbiology - 1 Nov 2006
Zhou Zhiyong, Poe Amanda C, Limor Josef, Grady Katharine K, Goldman Ira, McCollum Andrea M, Escalante Ananias A, Barnwell John W, Udhayakumar Venkatachalam
Abstract excerpt
A pyrosequencing protocol was developed as a rapid and reliable method to identify the mutations of the dhfr and dhps genes of Plasmodium falciparum that are associated with antifolate resistance. The accuracy and specificity of this method were tested using six laboratory-cultured P. falciparum...
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