Article
Can cloning and sequencing help to genotype positive Toxoplasma gondii clinical samples? Results and validation using SAG3 as a model.
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases - 1 Jul 2022
Rico-Torres Claudia Patricia, Valenzuela-Moreno Luis Fernando, Méndez-Cruz Sara Teresa, Cedillo-Peláez Carlos, Caballero-Ortega Heriberto
Abstract excerpt
Genotyping of T. gondii in human cases is relevant to understand the transmission patterns and epidemiology of this parasitosis. However, this genetic characterization can be hampered by the difficulty of isolating the parasite from mild or asymptomatic cases and by the detection efficiency of molecular assays such as the multilocus nested-polymerase chain reaction-restriction fragment length polymorphism...
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