Role of cysteines 640, 656, and 661 in steroid binding to rat glucocorticoid receptors.

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Article

Role of cysteines 640, 656, and 661 in steroid binding to rat glucocorticoid receptors.

The Journal of biological chemistry - 5 Jun 1992

Chakraborti P K, Garabedian M J, Yamamoto K R, Simons S S

Abstract excerpt

The involvement of a vicinally spaced dithiol group in steroid binding to the glucocorticoid receptor has been deduced from experiments with the thiol-specific reagent methyl methanethiolsulfonate and the vicinal dithiol-specific reagent sodium arsenite. The vicinally spaced dithiol appears to reside in the 16-kDa trypsin fragment of the receptor, which is thought to contain 3 cysteines (Cys-640, -656, and -661...

Topics

Affinity Labels
Animals
Arsenic
Arsenites
Base Sequence
Blotting, Western
Cysteine
Dexamethasone
Electrophoresis, Polyacrylamide Gel
Heat-Shock Proteins
Methyl Methanesulfonate

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Peptide Fragments

Rats

Receptors, Glucocorticoid

Steroids

Substrate Specificity

Source

Open in PubMed

Identifiers

PMID: 1597467

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1597467-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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