Identification of single amino acid substitutions of Cys-736 that affect the steroid-binding affinity and specificity of the glucocorticoid receptor using phenotypic screening in yeast.

Abstract excerpt

In this study a yeast-screening system has been developed for the isolation of rarely occurring change-of-function missense mutations in defined protein segments that have potential to give more information about the function of mutated residues. Mutagenesis of cysteine-736 was chosen for this in...

Topics

• Amino Acids
• Animals
• COS Cells
• Cysteine
• Humans
• Mammals
• Mutagenesis
• Mutation
• Phenotype
• Protein Engineering
• Receptors, Glucocorticoid
• Recombinant Proteins