Article
[Orange fluorescent protein--modification of green fluorescent protein GFPxm].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Jan 2003
Luo Wen-Xin, Chen Min, Cheng Tong, Guan Bao-Quan, Li Shao-Wei, Li Shao-Jing, Zhang Jun, Xia Ning-Shao
Abstract excerpt
Recently, we have reported a new gfp gene isolated from Aequorea macrodactyla. The protein purified from expressed E. coli exhibited an excitation peak at 476 nm and an emission peak at 496 nm. However, the drawback of only maturing to fluorescence at low temperature limited its applications. In this paper, we further describe twelve mutants of GFPxm. Seven mutants produced enhanced fluorescence when expressed in...
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