Article
A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteins.
FEBS letters - 30 Jan 2002
Yanushevich Yurii G, Staroverov Dmitry B, Savitsky Alexander P, Fradkov Arkady F, Gurskaya Nadya G, Bulina Maria E, Lukyanov Konstantin A, Lukyanov Sergey A
Abstract excerpt
Recently, we cloned several fluorescent proteins of different colors homologous to Aequorea victoria green fluorescent protein, which have great biotechnological potential as in vivo markers of gene expression. However, later investigations revealed severe drawbacks in the use of novel fluorescent proteins (FPs), in particular, the formation of tetramers (tetramerization) and high molecular weight aggregates...
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