Article
Trans-dominant inhibition of RNA viral replication can slow growth of drug-resistant viruses.
Nature genetics - 1 Jul 2005
Crowder Scott, Kirkegaard Karla
Abstract excerpt
The high error rates of viral RNA-dependent RNA polymerases create heterogeneous viral populations whose disparate RNA genomes affect each other's survival. We systematically screened the poliovirus genome and identified four sets of dominant mutations. Mutated alleles in capsid- and polymerase-coding regions resulted in dominant negative phenotypes, probably due to the proteins' oligomeric properties. We also...
Topics
- Alleles
- Antiviral Agents
- Capsid Proteins
- Cysteine Endopeptidases
- Drug Resistance, Viral
- Genome, Viral
- HeLa Cells
- Humans
- Isoxazoles
- Models, Molecular
- Mutation
- Phenotype
- Poliovirus
- Protein Biosynthesis
- Viral Proteins
- Virus Replication