Article
A sandwich enzyme-linked immunosorbent assay for human plasma apolipoprotein A-V concentration.
Journal of lipid research - 1 Sept 2005
Ishihara Mitsuaki, Kujiraoka Takeshi, Iwasaki Tadao, Nagano Makoto, Takano Mayumi, Ishii Jun, Tsuji Masahiro, Ide Hajime, Miller Irina P, Miller Norman E, Hattori Hiroaki
Abstract excerpt
Apolipoprotein A-V (apoA-V) is a recently discovered apolipoprotein that appears to have a role in plasma triglyceride (TG) transport. We have developed an ELISA for apoA-V using monoclonal antibodies that has a lower limit of detection of 0.3 ng/ml and linearity up to 20 ng/ml. The ELISA was then used to quantify plasma apoA-V in 196 healthy subjects and 106 patients with insulin-resistant diabetes mellitus. In...
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