Article
An enzyme-linked immunosorbent assay method to measure human apolipoprotein E levels using commercially available reagents: effect of apolipoprotein E polymorphism on serum apolipoprotein E concentration.
Analytical biochemistry - 1 Dec 1994
Gracia V, Fiol C, Hurtado I, Pintó X, Argimon J M, Castiñeiras M J
Abstract excerpt
A sensitive and specific enzyme-linked immunosorbent assay (ELISA) for human apolipoprotein E (apo E) quantification using commercially available reagents is described. The assay is a noncompetitive, sandwich ELISA in which the wells were coated with a monoclonal EO1 antibody anti-human apo E and...
Topics
- Adult
- Aged
- Alleles
- Apolipoprotein E2
- Apolipoprotein E3
- Apolipoprotein E4
- Apolipoproteins E
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Indicators and Reagents
- Lipids
- Male
- Middle Aged
- Phenotype