Article
Identification of sulI allele of dihydropteroate synthase by representational difference analysis in Haemophilus parasuis serovar 2.
Letters in applied microbiology - 1 Jan 2005
Del Rio M L, Navas-Mendez J, Gutierrez-Martin C B, Rodriguez-Barbosa J I, Rodriguez-Ferri E F
Abstract excerpt
AIMS: Identification of genes differentially present in Haemophilus parasuis serovar 2 by representational difference analysis (RDA). METHODS AND RESULTS: Bacterial genomic DNA was extracted, cleaved with Sau3AI and ligated to oligonucleotide adapter pair. The optimal tester (H. parasuis serovar 2)/driver ratio (H. parasuis serovars 1, 3 and 5) for the hybridization was established and the mixture was hybridized,...
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