Article
aroA gene PCR-RFLP diversity patterns in Haemophilus parasuis and Actinobacillus species.
Research in veterinary science - 1 Feb 2006
del Río M L, Martín C B Gutiérrez, Navas J, Gutiérrez-Muñiz B, Rodríguez-Barbosa J I, Rodríguez Ferri E F
Abstract excerpt
The Haemophilus parasuis aroA gene encodes 5-enolpyruvylshikimate-3-phosphate synthase and participates in the aromatic amino acids and the folic acid universal metabolic pathway of bacteria. The application of aroA-based PCR-RFLP methodology yields a significant degree of diversity in H. parasuis and Actinobacillus species. PCR amplification of the aroA gene rendered a 1,067-bp fragment in all 15 H. parasuis...
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