Rapid and efficient screening of a Representational Difference Analysis library using reverse Southern hybridisation: identification of genetic differences between Haemophilus parasuis isolates.

Abstract excerpt

Representational Difference Analysis (RDA) is an established technique used for isolation of specific genetic differences between or within bacterial species. This method was used to investigate the genetic basis of serovar-specificity and the relationship between serovar and virulence in Haemophilus parasuis. An RDA clone library of 96 isolates was constructed using H. parasuis strains H425(P) (serovar 12) and...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Blotting, Southern
• Share to DNA, Bacterial