Article
Direct detection in clinical samples of multiple gene mutations causing resistance of Mycobacterium tuberculosis to isoniazid and rifampicin using fluorogenic probes.
The Journal of antimicrobial chemotherapy - 1 Jun 2005
Espasa Mateu, González-Martín Julián, Alcaide Fernando, Aragón Lina Marcela, Lonca Joan, Manterola Joxe Mari, Salvadó Margarita, Tudó Griselda, Orús Pilar, Coll Pere
Abstract excerpt
BACKGROUND: This study evaluates a method based on real-time PCR for direct detection in clinical samples of the common mutations responsible for isoniazid and rifampicin resistance of Mycobacterium tuberculosis. METHODS: Six pairs of fluorogenic 5' exonuclease probes (Taqman), mutated and wild-type, were designed for six targets: codon 315 of katG, substitution C209T in the regulatory region of inhA, and codons...
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