Article
New multiplex PCR for rapid detection of isoniazid-resistant Mycobacterium tuberculosis clinical isolates.
Antimicrobial agents and chemotherapy - 1 Jan 2005
Herrera-León Laura, Molina Tamara, Saíz Pilar, Sáez-Nieto Juan Antonio, Jiménez Maria Soledad
Abstract excerpt
In this study, we describe a multiplex PCR to detect a AGC-->ACC (serine to threonine) mutation in the katG gene and a -15 C-to-T substitution (inhA(C-15T)) at the 5' end of a presumed ribosome binding site in the promoter of the mabA-inhA operon. These mutations have been reported in the majority of previous studies as the most frequent mutations involved in the resistance to isoniazid (INH) of Mycobacterium...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.