Article
Tryptophan to phenylalanine substitutions allow differentiation of short- and long-range conformational changes during denaturation of goat alpha-lactalbumin.
Proteins - 1 Jul 2005
Vanhooren Ann, Chedad Allel, Farkas Viktor, Majer Zsuzsa, Joniau Marcel, Van Dael Herman, Hanssens Ignace
Abstract excerpt
To test the occurrence of local particularities during the unfolding of Ca2+-loaded goat alpha-lactalbumin (GLA) we replaced Trp60 and -118, either one or both, by Phe. In contrast with alternative studies, our recombinant alpha-lactalbumins are expressed in Pichia pastoris and do not contain the extra N-terminal methionine. The substitution of Trp60 leads to a reduction of the global stability. The effect of the...
Topics
- Amino Acid Substitution
- Animals
- Calorimetry, Differential Scanning
- Circular Dichroism
- Goats
- Kinetics
- Lactalbumin
- Milk
- Mutation
- Phenylalanine
- Protein Conformation
- Protein Denaturation
- Protein Folding