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Article

Spectroscopic analysis of a methionine-48 to tyrosine mutant of chicken troponin C.

Biochemistry - 13 Oct 1992

Pearlstone J R, McCubbin W D, Kay C M, Sykes B D, Smillie L B

Abstract excerpt

A mutant (M48Y) of chicken skeletal muscle troponin C was prepared in which Tyr replaced Met-48 of the recombinant protein (rTnC). Since Tyr and Trp are normally absent, spectral properties could be unambiguously assigned to the site of substitution. In the crystal structure, this residue lies at the COOH-terminal end of the B-helix of the N domain in a region postulated to undergo a significant conformational...

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Identifiers

PMID: 1390746
DOI: 10.1021/bi00155a025

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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