Spectroscopic analysis of a methionine-48 to tyrosine mutant of chicken troponin C.

doi:10.1021/bi00155a025

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Article

Spectroscopic analysis of a methionine-48 to tyrosine mutant of chicken troponin C.

Biochemistry - 13 Oct 1992

Pearlstone J R, McCubbin W D, Kay C M, Sykes B D, Smillie L B

Abstract excerpt

A mutant (M48Y) of chicken skeletal muscle troponin C was prepared in which Tyr replaced Met-48 of the recombinant protein (rTnC). Since Tyr and Trp are normally absent, spectral properties could be unambiguously assigned to the site of substitution. In the crystal structure, this residue lies at...

Topics

Animals
Base Sequence
Calcium
Cations, Divalent
Chickens
Circular Dichroism
Hydrogen-Ion Concentration
Magnetic Resonance Spectroscopy
Methionine
Molecular Sequence Data
Mutation
Oligonucleotides
Spectrophotometry, Ultraviolet
Troponin
Troponin C
Tyrosine

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