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Article

Mutations in the "lid" region affect chain length specificity and thermostability of a Pseudomonas fragi lipase.

FEBS letters - 25 Apr 2005

Santarossa Gianluca, Lafranconi Pietro Gatti, Alquati Claudia, DeGioia Luca, Alberghina Lilia, Fantucci Piercarlo, Lotti Marina

Abstract excerpt

The cold-adapted Pseudomonas fragi lipase (PFL) displays highest activity on short-chain triglyceride substrates and is rapidly inactivated at moderate temperature. Sequence and structure comparison with homologous lipases endowed with different substrate specificity and stability, pointed to three polar residues in the lid region, that were replaced with the amino acids conserved at equivalent positions in the...

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Identifiers

PMID: 15848176
DOI: 10.1016/j.febslet.2005.03.037

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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