Article
Identification of cis-regulatory sequences in the human angiotensinogen gene by transgene coplacement and site-specific recombination.
Molecular and cellular biology - 1 Apr 2005
Shimizu Taku, Oishi Takayuki, Omori Akane, Sugiura Akiko, Hirota Keiko, Aoyama Hisanori, Saito Tomoko, Sugaya Takeshi, Kon Yasuhiro, Engel James Douglas, Fukamizu Akiyoshi, Tanimoto Keiji
Abstract excerpt
The function of putative regulatory sequences identified in cell transfection experiments can be elucidated only through in vivo experimentation. However, studies of gene regulation in transgenic mice (TgM) are often compromised by the position effects, in which independent transgene insertions differ in expression depending on their location in the genome. In order to overcome such a dilemma, a method called...
Topics
- Angiotensinogen
- Animals
- Base Sequence
- Cell Line
- Chromosomes, Artificial, Bacterial
- Down-Regulation
- Gene Targeting
- Humans
- Integrases
- Mice