Article
Helical crystallization on lipid nanotubes: streptavidin as a model protein.
Journal of structural biology - 1 Apr 2005
Dang Thanh X, Farah Sammy J, Gast Alice, Robertson Channing, Carragher Bridget, Egelman Edward, Wilson-Kubalek Elizabeth M
Abstract excerpt
In this study, we use streptavidin (SA) as a model system to study helical protein array formation on lipid nanotubes, an alternative to 2D studies on lipid monolayers. We demonstrate that wild-type and a mutant form of SA form helical arrays on biotinylated lipid nanotubes. 3D maps from helical arrays of wild-type and mutant SA were reconstructed using two different approaches: Fourier-Bessel methods and an...
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